sterilization means that destroy all the life forms including the fungi,bacteria,viruses, endospores.There are several ways to do this processthe basic principle of the steam sterilization is that we should consider pressure time and temperature.The steam sterilization have its specific pressure,time and temperature that can reach the purpose.
there are two major type of the autoclave:
first is pull off all the air by vacuum pump
second is also pull off the air by gravity
What the action of the steam sterilization on the microbes????The moist can destroy its protein or enzyme
The critical and semicrical item with moist and heat resistant can be used
relevant video about steam sterilization:https://www.youtube.com/watch?v=Y2JUkRsYjIM

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