Factors Affecting the Efficacy of Disinfection and Sterilization

1.we need the more time or higher level germicide if the number of microbes is higher

2.the level of germicide will affect the time or efficacy.High level germicide can destroy the microbes more effective

3.some microbes have resistance to the germicide,maybe innate or acqired

4.some environment factor can affect the efficacy such as PH,temperature .......and so on

5.depend on how long does the mircobes exposure in the germicides

 Chemical disinfectants

1.household bleach

mode of action:decrease DNA and protein synthesis and decreased uptake of nutrition

uses:to clean the item in the house

2.Glutaraldehyde

 mode of action:change and affect the protein,DNA and RNA synthesis of the microbes


uses:as a high level disinfectant on the instrument.